ESR has developed a MLPA assay that rapidly detects the genes important for Shiva toxin-producing E. coli (STEC) including the seven serotypes important to exporters of red meat to North America (e.g. E. coli HO157:H7). In addition ESR has also developed two PCR-based sub-typing assays (P-BIT), one for STEC and one for Campylobacter. There is significant international interest in these sub-typing assays but they currently require large numbers (18 for Campylobacter and 24 for STEC) of individual PCR reactions to produce a type profile. The conversion of these P-BIT assays to MLPA allows all of the targets to be detected in a single reaction. ESR has designed the probes for 38 E. coli and 14 Campylobacter gene targets to date. The genes have been chosen from published papers using gene sequences that are publicly available. The specific sequences of the probes have been chosen by ESR (for the STEC-Super 6 [12 probe sets], STEC P-BIT [24 probe sets], Campy P-BIT [14 probe sets] )
ESR is interested in working with parties interested in the identification of food borne pathogens with a view to further validating the assays and ultimately using them in a commercial sense.
Currently protected by trade secret although some opportunity exists to patent specific aspects of the technology
Proof of Principle - Laboratory demonstration of principle only